The multimodal cation exchanger ligand of Capto MMC ImpRes is immobilized to the base matrix and interacts with the target molecule through multiple types of interactions. The weak cation exchange multimodal ligand enables high selectivity in a broad pH/salt window compared with traditional ion exchangers, which allows the use of Capto MMC ImpRes in a variety of process conditions to solve challenging purification problems. Suitable for polishing of antibody fragments.Displays a broad pH/salt operational window.Enables use of a platform approach to MAb process development.Efficient removal of aggregates, viruses, and main contaminants in MAb processes.Burgess RR (2018) A brief practical review of size exclusion chromatography: rules of thumb, limitations, and troubleshooting.Arakawa T, Ejima D, Tsumoto K, Obeyama N, Tanaka Y, Kita Y, Timasheff SN (2007) Suppression of protein interactions by arginine: a proposed mechanism of the arginine effects.GE Healthcare Life Sciences (2015) Capto™ MMC ImpRes.GE Healthcare Life Sciences (2013) Polishing of monoclonal antibodies using Capto™ MMC ImpRes in bind and elute mode.Chmielowski RA, Meissner S, Roush D, Linden TO, Glowacki E, Konietzko J, Nti-Gyabaah J (2014) Resolution of heterogeneous charged antibody aggregates via multimodal chromatography: a comparison to conventional approaches.Gao D, Wang LL, Lin DQ, Yao SJ (2013) Evaluating antibody monomer separation from associated aggregates using mixed-mode chromatography.Chen J, Tetrault J, Zhang Y, Wasserman A, Conley G, Dileo M, Haimes E, Nixon AE, Ley A (2010) The distinctive separation attributes of mixed-mode resins and their application in monoclonal antibody downstream purification process.Eriksson K, Ljunglöf A, Rodrigo G, Brekkan E (2009) MAb contaminant removal with a multimodal anion exchanger: a platform step to follow protein A.Gagnon P, Beam K (2009) Antibody aggregate removal by hydroxyapatite chromatography.Gagnon P, Ng P, Aberrin C, Zhen J, He J, Mekosh H, Cummings L, Richieri R, Zaidi S (2006) A ceramic hydroxyapatite based purification platform: simultaneous removal of leached protein A, aggregates, DNA, and endotoxins.McCue JT (2009) Theory and use of hydrophobic interaction chromatography in protein purification applications.Lu Y, Williamson B, Gillespie R (2009) Recent advancement in application of hydrophobic interaction chromatography for aggregate removal in industrial purification process.McCue JT, Engel P, Ng A, Macniven R, Thömmes J (2008) Modeling of protein monomer/aggregate purification and separation using hydrophobic interaction chromatography.Zhang X, Chen T, Li Y (2019) A parallel demonstration of different resins' antibody aggregate removing capability by a case study.He X (2015) Mixed-mode chromatography for mAb S aggregate removal: comparison of CHT™ ceramic hydroxyapatite, capto adhere, and capto adhere impres.Hall T, Wilson JJ, Brownlee TJ, Swartling JR, Langan SE, Lambooy PK (2015) Alkaline cation-exchange chromatography for the reduction of aggregate and a mis-formed disulfide variant in a bispecific antibody purification process.Vázquez-Rey M, Lang DA (2011) Aggregates in monoclonal antibody manufacturing processes.Bonnerjea J, Brake RP, Davis MR, Kellerman K, Preneta A (2008) Ion exchange chromatography and purification of antibodies, United States patent application publication US 2008/0312425 A1.
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